abstract：:According to a basic formulation of the quantal model, evoked synaptic currents are made up of a linear summation of uniquantal synaptic currents, which in turn are equivalent to the spontaneous miniature synaptic currents ('minis') that often persist when evoked neurotransmitter release is blocked. Here I describe a ...

journal_title：Journal of neuroscience methods

authors： Bekkers JM

更新日期：2003-12-15 00:00:00

abstract：:Display systems based on conventional computer graphics cards are capable of generating images with about 8-bit luminance resolution. However, most vision experiments require more than 12 bits of luminance resolution. Pelli and Zhang [Spatial Vis. 10 (1997) 443] described a video attenuator for generating high luminan...

journal_title：Journal of neuroscience methods

authors： Li X,Lu ZL,Xu P,Jin J,Zhou Y

更新日期：2003-11-30 00:00:00

abstract：:The influence of direct electric stimulation on nerve regeneration was studied in a model of crush injury of the sciatic nerve of rats. Forty-three rats were used and distributed in four groups according to the procedure: (1) intact nerve, inactive circuit; (2) crush injury, inactive circuit; (3) intact nerve, active ...

journal_title：Journal of neuroscience methods

authors： Mendonça AC,Barbieri CH,Mazzer N

更新日期：2003-10-30 00:00:00

abstract：:Chronic spinal catheterization via an atlanto-occipital puncture (CAO) has been widely used to study the effects of drugs on spinal nociceptive mechanisms, but this method is associated with spinal cord damage that may change the efficacy of spinally injected analgesics. Using a slight modification of the method of St...

journal_title：Journal of neuroscience methods

authors： Prado WA

更新日期：2003-10-15 00:00:00

abstract：:Slice cultures on biomembrane are the method of choice for studying Ca2+-dependent plastic changes occurring over several days to weeks. Using IR-differential interference contrast, good visualization of neurons in biomembrane slice cultures has been achieved despite a negative optical effect of the biomembrane, but e...

journal_title：Journal of neuroscience methods

authors： Alix P,Winterer J,Müller W

更新日期：2003-09-30 00:00:00

abstract：:Neurons growing on microelectrode arrays (MEAs) are promising tools to investigate principal neuronal network mechanisms and network responses to pharmaceutical substances. However, broad application of these tools, e.g. in pharmaceutical substance screening, requires neuronal cells that provide stable activity on MEA...

journal_title：Journal of neuroscience methods

authors： Otto F,Görtz P,Fleischer W,Siebler M

更新日期：2003-09-30 00:00:00

abstract：:A double pipette system for local, controlled drug infusion is presented. Two concentric pipettes can be manipulated separately and pressurized independently by a designated double holder. The inner pipette is loaded with the desirable solution (drug), and functions as a source, while the outer one is a sink. This giv...

journal_title：Journal of neuroscience methods

authors： Feinerman O,Moses E

更新日期：2003-07-15 00:00:00

abstract：:The reproducibility of motor unit twitches obtained using spike-triggered averaging (STA) was examined in the human first dorsal interosseus. For each motor unit (30 total) a series of STA twitches was derived using a 30 s averaging window. Within each averaging window, eight independent measures characterizing motor ...

journal_title：Journal of neuroscience methods

authors： Gossen ER,Ivanova TD,Garland SJ

更新日期：2003-06-30 00:00:00

abstract：:Depression is characterized by a lack of "motivation" rather than a lack of "physical space" to move around. This study was designed to evaluate predictivity of an open space swimming test for antidepressant activity of various antidepressants in rats. Without drug treatment, rats showed a significant reduction in the...

journal_title：Journal of neuroscience methods

authors： Sun MK,Alkon DL

更新日期：2003-06-15 00:00:00

abstract：:We demonstrate herein that the standard 'Trolox equivalent antioxidant capacity' (TEAC) assay, typically utilized to quantify total antioxidant levels within plasma, can also be utilized for tissue homogenates. Normal mice and transgenic mice lacking apolipoprotein E were subjected to a diet including iron as a generi...

journal_title：Journal of neuroscience methods

authors： Shea TB,Rogers E,Ashline D,Ortiz D,Sheu MS

更新日期：2003-05-30 00:00:00

abstract：:The most commonly used method for specifying the locations of functional areas in the human cerebral cortex is the coordinate system of Talairach and Tournoux (Co-planar Stereotaxic Altas of The Human Brain (1988) Georg Thieme Verlag, Stuttgart). It was designed to locate subcortical nuclei by reference to an axis joi...

journal_title：Journal of neuroscience methods

authors： Li DF,Freeman AW,Tran-Dinh H,Morris JG

更新日期：2003-05-30 00:00:00

abstract：:We present a numerical method which provides the ability to analyze digitized microscope images of retinal explants and quantify neurite outgrowth. Few parameters are required as input and limited user interaction is necessary to process an entire experiment of images. This eliminates fatigue related errors and user-r...

journal_title：Journal of neuroscience methods

authors： Weaver CM,Pinezich JD,Lindquist WB,Vazquez ME

更新日期：2003-04-15 00:00:00

abstract：:Epoxy-insulated tungsten microelectrodes can be used once or twice in our lab before the impedance becomes too low. Dipping the electrodes in epoxy followed by curing restores their initial high impedance which is associated with good isolation of single neurons. It is a cost effective and simple procedure. ...

journal_title：Journal of neuroscience methods

authors： Verhagen JV,Gabbott PL,Rolls ET

更新日期：2003-03-15 00:00:00

abstract：:Characterizing the structure and electrophysiological properties of single neurons is essential for understanding how individual cells contribute to the function of neuronal networks. Following intra-cellular recording from neurons in acute brain slices, the structure of the recorded cell has typically been examined b...

journal_title：Journal of neuroscience methods

authors： Hamam BN,Kennedy TE

更新日期：2003-02-15 00:00:00

abstract：:The concentration of free calcium within the lumen of the endoplasmic reticulum ([Ca2+]L) fluctuates between 100 and 1000 microM. High [Ca2+]L provides an electro-driving force for Ca2+ release and supports high Ca2+ diffusion rate within the endoplasmic reticulum lumen. Fluctuations in [Ca2+]L also regulate numerous ...

journal_title：Journal of neuroscience methods

authors： Solovyova N,Verkhratsky A

更新日期：2002-12-31 00:00:00

abstract：:Controlled expression of proteins is a key experimental approach to a deeper understanding of the molecular basis of neuronal function. Here we evaluate the HSV-1 (herpes simplex virus) amplicon vector for gene delivery into the brains of living rats. We demonstrate that HSV-1 amplicon vectors expressing enhanced gree...

journal_title：Journal of neuroscience methods

authors： Sandler VM,Wang S,Angelo K,Lo HG,Breakefield XO,Clapham DE

更新日期：2002-12-15 00:00:00

abstract：:Previously, we developed technology that coupled high-speed chronoamperometry with microejections of dopamine (DA) to measure DA clearance in the brains of freely-behaving rats. Here, by varying the ejection volumes of DA across a 200-fold difference, the kinetics of striatal clearance were analyzed as a function of t...

journal_title：Journal of neuroscience methods

authors： Sabeti J,Adams CE,Burmeister J,Gerhardt GA,Zahniser NR

更新日期：2002-11-15 00:00:00

abstract：:This work demonstrates technical approaches to high-quality magnetic resonance imaging (MRI) of small structures of the mouse brain in vivo. It turns out that excellent soft-tissue contrast requires the reduction of partial volume effects by using 3D MRI at high (isotropic) resolution with linear voxel dimensions of a...

journal_title：Journal of neuroscience methods

authors： Natt O,Watanabe T,Boretius S,Radulovic J,Frahm J,Michaelis T

更新日期：2002-10-30 00:00:00

abstract：:Hippocampal slices were obtained from hippocampi of patients with temporal lobe epilepsy or from patients with mass lesions located in the temporal lobe. Hippocampal slices were kept alive in a slice chamber and the anterograde tracer neurobiotin was iontophoretically injected into the granule cell layer of the dentat...

journal_title：Journal of neuroscience methods

authors： Freiman TM,Gimbel K,Honegger J,Volk B,Zentner J,Frotscher M,Deller T

更新日期：2002-10-15 00:00:00

abstract：:This paper describes improvements and further characterization of a ceramic-based multisite microelectrode for in vivo measurements of L-glutamate. Improvements include increased recording area, insulation deposition using photolithography for more uniform recording sites and forming the microelectrodes using a diamon...

journal_title：Journal of neuroscience methods

authors： Burmeister JJ,Pomerleau F,Palmer M,Day BK,Huettl P,Gerhardt GA

更新日期：2002-09-30 00:00:00

abstract：:The thalamocortical slice is widely employed for in vitro studies of cortical circuits. This preparation was developed in order to preserve anatomical and functional connectivity between the ventrobasal thalamus and somatosensory (whisker/barrel) cortex of young mice, and thalamocortical slice experiments have contrib...

journal_title：Journal of neuroscience methods

authors： Land PW,Kandler K

更新日期：2002-09-15 00:00:00

abstract：:Odor input evokes characteristic, time-evolving (non-stationary) events in the spontaneously active central ganglia of the snail Helix pomatia. Assuming stationarity for the signals, one could, as the first approach, apply the Fourier-based methods, frequency amplitude characteristics (FAC) measures, for analyzing suc...

journal_title：Journal of neuroscience methods

authors： Schütt A,Rosso OA,Figliola A

更新日期：2002-09-15 00:00:00

abstract：:A reliable focal cerebral ischemia model is essential in evaluating the efficacy and safety of neuroprotective therapy for stroke. We present a focal embolic ischemic model of rat with reproducible and predictable brain infarction by comparing it with two other most commonly used focal cerebral ischemia models. To pro...

journal_title：Journal of neuroscience methods

authors： Yang Y,Yang T,Li Q,Wang CX,Shuaib A

更新日期：2002-08-30 00:00:00

abstract：:A low-noise flexible system for the simultaneous recording and analysis of several electrical signals (EEG, ECG, EMG, and diaphragm EMG) from the same rat was constructed for studying changes in physiological functions during the sleep-wake cycle. The hardware in the system includes a multichannel amplifier, a video c...

journal_title：Journal of neuroscience methods

authors： Shaw FZ,Lai CJ,Chiu TH

更新日期：2002-07-30 00:00:00

abstract：:Inefficiency in primary neural stem cell transfection is a major obstacle in gene expression research aimed at determining the mechanisms underlying brain development. Following our initial finding that liposome-mediated transfection procedures are fairly toxic to neural stem cells, we further examined whether transfe...

journal_title：Journal of neuroscience methods

authors： Kim YC,Shim JW,Oh YJ,Son H,Lee YS,Lee SH

更新日期：2002-06-30 00:00:00

abstract：:The detection of extracellular potentials by means of multi-electrode arrays (MEA) is a useful technique for multi-site long-term monitoring of cultured neuronal activity with single-cell resolution. To optimize the geometry of the MEA it is advantageous to localize the cellular compartments that constitute the genera...

journal_title：Journal of neuroscience methods

authors： Claverol-Tinture E,Pine J

更新日期：2002-05-30 00:00:00

abstract：:Scanning ion conductance microscopy (SICM) takes advantage of the increase in the resistance which occurs if a glass microelectrode is closely approached to a poorly conducting membrane (Science 243 (1989) 641) and has been shown to be a promising technique to study membranes of living cells (Biophys J 73 (1997a) 653;...

journal_title：Journal of neuroscience methods

authors： Mann SA,Hoffmann G,Hengstenberg A,Schuhmann W,Dietzel ID

更新日期：2002-05-15 00:00:00

abstract：:To facilitate the combination of optical imaging with various electrode-based techniques, we have designed and produced a skull-mounting "sliding-top cranial window" and a removable "electrode positioner microdrive". These new devices were used to study sensory processing in chronic and acute experiments in the cerebr...

journal_title：Journal of neuroscience methods

authors： Arieli A,Grinvald A

更新日期：2002-04-30 00:00:00

abstract：:Much interest has been shown in the use of multi-template reverse transcription-polymerase chain reaction (RT-PCR) as a quantitative instrument for low-abundance mRNAs. A desire to achieve finely-graded quantification of the stress- and hormone-related regulation of one splicing decision in an ion channel gene motivat...

journal_title：Journal of neuroscience methods

authors： Mahmoud SF,Bezzerides AL,Riba R,Lai GJ,Lovell PV,Hara Y,McCobb DP

更新日期：2002-04-15 00:00:00

abstract：:Voltage-sensitive dyes (VSDs) and optical imaging are useful for studying spatiotemporal patterns of population neuronal activity in cortical tissue. Using a photodiode array and absorption dyes we were able to detect neuronal activity in single trials before it could be detected by local field potential (LFP) recordi...

journal_title：Journal of neuroscience methods

authors： Jin W,Zhang RJ,Wu JY

更新日期：2002-03-30 00:00:00

abstract：:Since pain is an important symptom in arthritis, useful behavioral indices for pain in experimental arthritis animal models are important tools for investigative work on arthritis. The purpose of this study was to develop simple and quantifiable behavioral tests, which would represent the level of arthritic pain that ...

journal_title：Journal of neuroscience methods

authors： Yu YC,Koo ST,Kim CH,Lyu Y,Grady JJ,Chung JM

更新日期：2002-03-30 00:00:00

abstract：:A video-based 2D eye-tracking system (EyeLink version 2.04, SR Research Ltd/SMI) was compared with the scleral search coil technique for its performance on recording the properties of fixations and saccadic eye movements. Fixation positions and saccadic properties (amplitude, duration, and peak velocity) were calculat...

journal_title：Journal of neuroscience methods

authors： van der Geest JN,Frens MA

更新日期：2002-03-15 00:00:00

abstract：:We describe the fabrication and use of a new type of extracellular micro-electrode array mounted on a flexible transparent polyimide substrate that can be rapidly moved from one part of a culture of vertebrate neurons (rat nodose) to another, which permits co-culture of glia under the neurons and is easily and rapidly...

journal_title：Journal of neuroscience methods

authors： Sandison M,Curtis AS,Wilkinson CD

更新日期：2002-02-15 00:00:00

abstract：:We present a new analytical method for characterizing the directional tuning of neural data. The method is based on computing parameters associated with the geometric properties of solids, and provides an estimate of preferred direction in the context of non-uniform sampling of directions. Unlike optimization methods ...

journal_title：Journal of neuroscience methods

authors： Gribble PL,Scott SH

更新日期：2002-01-30 00:00:00

abstract：:Optical imaging over extended periods of time in non-human primates presents serious challenges because the dura mater must be removed to expose the cortical surface. We present a novel nylon imaging chamber with a transparent artificial dura implant, which allows repeated, long-term optical recordings from the cortex...

journal_title：Journal of neuroscience methods

authors： Chen LM,Heider B,Williams GV,Healy FL,Ramsden BM,Roe AW

更新日期：2002-01-15 00:00:00

abstract：:Electroneurograms (ENGs) from the vagus, splanchnic and pancreatic nerves innervating the pancreas of a dog, were recorded with chronically implanted silicone multi-electrode circular cuffs in an intact pancreas and in a pancreas partly disabled with alloxan. The cuffs contained 33 platinum electrodes (0.6x1.5 mm) arr...

journal_title：Journal of neuroscience methods

authors： Rozman J,Zorko B,Bunc M

更新日期：2001-12-15 00:00:00

abstract：:We describe a method of interactive three-dimensional segmentation and visualization for anatomical magnetic resonance imaging (MRI) data in a personal computer environment. The visual feedback necessary during 3-D segmentation was provided by a ray casting algorithm, which was designed to allow users to interactively...

journal_title：Journal of neuroscience methods

authors： Yoo SS,Lee CU,Choi BG,Saiviroonporn P

更新日期：2001-11-15 00:00:00

abstract：:Evaluation of electron microscopic images of serial sections is a time-consuming process requiring a high level of expertise. Here we present an algorithm to ease and accelerate this process. It is a modification of an algorithm successfully used in computer vision for object recognition. However, rather than recognis...

journal_title：Journal of neuroscience methods

authors： König P,Kayser C,Bonin V,Würtz RP

更新日期：2001-10-30 00:00:00

abstract：:We describe here an experimental approach designed to aid in the identification of complex brain circuits within the rat corpus striatum. Our aim was to characterize in a single section (i) striatal thalamic afferents, (ii) striatopallidal projection neurons and (iii) striatal local circuit interneurons. To this end, ...

journal_title：Journal of neuroscience methods

authors： Gonzalo N,Moreno A,Erdozain MA,García P,Vázquez A,Castle M,Lanciego JL

更新日期：2001-10-15 00:00:00

abstract：:We have developed a new method for culturing cells that maintains their health and sterility for many months. Using conventional techniques, primary neuron cultures seldom survive more than 2 months. Increases in the osmotic strength of media due to evaporation are a large and underappreciated contributor to the gradu...

journal_title：Journal of neuroscience methods

authors： Potter SM,DeMarse TB

更新日期：2001-09-30 00:00:00